Common issues to check

peterjack

If your 12, 14 or 16-bit images look all black when you open them, typically the problem is that the pixel values are very, very small relative to the maximum possible pixel value (4095, 16383, and 65535, respectively), so when displayed the pixels are effectively black. In ImageJ/Fiji, this is fixable by checking the “Autoscale” option; with the command line tools, the “-autoscale -fast” options should work.

Shatojon

Thank you, peter.